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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- ALX-803-081-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-803-081-C100, RRID:AB_2051772
- Product name
- LC3B monoclonal antibody (2G6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Thiophilic adsorption and size exclusion chromatography purified.
- Reactivity
- Human, Mouse, Rat, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G6
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis of endogenous LC3 using MAb to LC3B (Prod. No. ALX-803-081). Method: Whole cell lysates of untreatedÊ tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed withÊMonoclonal Antibody toÊLC3Ê(2G6) (Prod. No. ALX-803-081)Ê (0.5 µg/ ml) for 1h at RT and developed by ECL (exposure time: 30 sec).Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12Lane 7: CMT Lane 8: Neuro2A Lane 9: NIH - 3T
