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- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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- Product number
- GTX115098 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115098, RRID:AB_10624724
- Product name
- Liprin alpha 1 antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery.
Α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.
Bernadzki KM, Gawor M, Pęziński M, Mazurek P, Niewiadomski P, Rędowicz MJ, Prószyński TJ
Scientific reports 2017 Aug 22;7(1):9116
Scientific reports 2017 Aug 22;7(1):9116
Α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.
Gingras J, Gawor M, Bernadzki KM, Grady RM, Hallock P, Glass DJ, Sanes JR, Proszynski TJ
Journal of cell science 2016 Mar 1;129(5):898-911
Journal of cell science 2016 Mar 1;129(5):898-911
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Liprin alpha 1 antibody [N1N2], N-term detects PPFIA1 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract5 % SDS-PAGELiprin alpha 1 antibody [N1N2], N-term (GTX115098) dilution: 1:1000
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 5% SDS PAGE GTX115098 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Liprin alpha 1 antibody detects Liprin alpha 1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Liprin alpha 1 antibody (GTX115098) diluted by 1:1000.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein at cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Liprin alpha 1 protein stained by Liprin alpha 1 antibody [N1N2], N-term (GTX115098) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
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- GeneTex (provider)
- Main image
- Experimental details
- Liprin alpha 1 antibody [N1N2], N-term detects Liprin alpha 1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Liprin alpha 1 stained by Liprin alpha 1 antibody [N1N2], N-term (GTX115098) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Immunoprecipitation of Liprin alpha 1 protein from A431 whole cell extracts using 5 £gg of Liprin alpha 1 antibody [N1N2], N-term (GTX115098).Western blot analysis was performed using Liprin alpha 1 antibody [N1N2], N-term (GTX115098).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
