12900-1-AP

antibody from Invitrogen Antibodies

Targeting: RCAN2 DSCR1L1, ZAKI-4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12900-1-AP

Provider

Invitrogen Antibodies

Product name

RCAN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LFRTYDDCV TFQLFKSFRR VRINFSNPKS AARARIELHE TQFRGKKLKL YFAQVQTPET DGDKLHLAPP QPAKQFLISP PSSPPVGWQP INDATPVLNY DLLYAVAKLG PGEKYELHAG TESTPSVVVH VCDSDIEEEE DPKTSPKPKI IQTRRPGLPP SVSNWAAC (35-201 aa encoded by BC038509 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 12900-1-AP (RCAN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 12900-1-AP (RCAN2 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12900-1-AP ( RCAN2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12900-1-AP ( RCAN2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RCAN2 (IP:12900-1-AP, 4ug; Detection:12900-1-AP 1:300) with mouse brain tissue lysate 4400 ug.