NB100-79794
antibody from Novus Biologicals
Targeting: SPAG9
CT89, FLJ13450, FLJ14006, FLJ26141, FLJ34602, HLC4, HSS, JIP-4, JIP4, JLP, KIAA0516, MGC117291, MGC14967, MGC74461, PHET, PIG6, SYD1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB100-79794 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79794, RRID:AB_1109080
- Product name
- Rabbit Polyclonal SPAG9 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPAG9 Antibody [NB100-79794] - Detection of Human JLP by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and 293T (50ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPAG9 Antibody [NB100-79794] - Nuclear extract (NE, 20 ug) from HeLa cells. Antibody used at 0.3 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPAG9 Antibody [NB100-79794] - Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50 ug), Renca (50 ug), and CT26 (50 ug) cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SPAG9 Antibody [NB100-79794] - Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody NB100-79794 (lot NB100-79794-2) used for IP at 6 ug/mg lysate. JLP was also immunoprecipitated by a previous lot (NB100-79794-1) of this antibody. For blotting immunoprecipitated JLP, NB100-79794 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: SPAG9 Antibody [NB100-79794] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP used at a dilution of 1:1,000 (1ug/ml). Detection: DAB