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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4743 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4743, RRID:AB_1576976
- Product name
- MAGEA12 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAGEA12.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Comparative genome sequence analysis of the Bpa/Str region in mouse and Man.
The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Cloning and analysis of MAGE-1-related genes.
Mallon AM, Platzer M, Bate R, Gloeckner G, Botcherby MR, Nordsiek G, Strivens MA, Kioschis P, Dangel A, Cunningham D, Straw RN, Weston P, Gilbert M, Fernando S, Goodall K, Hunter G, Greystrong JS, Clarke D, Kimberley C, Goerdes M, Blechschmidt K, Rump A, Hinzmann B, Mundy CR, Miller W, Poustka A, Herman GE, Rhodes M, Denny P, Rosenthal A, Brown SD
Genome research 2000 Jun;10(6):758-75
Genome research 2000 Jun;10(6):758-75
The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Rogner UC, Wilke K, Steck E, Korn B, Poustka A
Genomics 1995 Oct 10;29(3):725-31
Genomics 1995 Oct 10;29(3):725-31
Cloning and analysis of MAGE-1-related genes.
Ding M, Beck RJ, Keller CJ, Fenton RG
Biochemical and biophysical research communications 1994 Jul 15;202(1):549-55
Biochemical and biophysical research communications 1994 Jul 15;202(1):549-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of K-562 cell lysate (35 ug/lane) with MAGEA12 polyclonal antibody (Cat # PAB4743).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAGEA12 polyclonal antibody (Cat # PAB4743) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
