PA5-96943

antibody from Invitrogen Antibodies

Targeting: MEP1A PPHA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96943

Provider

Invitrogen Antibodies

Product name

MEP1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: NFSQVLENTS KGDKLQSPRF YNSEGYGFGV TLYPNSRESS GYLRLAFHVC SGENDAILEW PVENRQVIIT ILDQEPDVRN RMSSSMVFTT SKSHTSPAIN DTVIWDRPSR VGTYHTDCNC FRSIDLGWSG FISHQMLKRR SFLKNDDLII FVDFEDITHL SQTEVPTKGK RLSPQGLILQ GQEQQVSEEG SGKAMLEEAL PVSLSQGQPS RQKRSVENTG PLEDHNWPQY FRDPCDPNPC QNDGICVNVK GMASCRCISG H; Positive Samples: Mouse kidney, Mouse small intestine, Mouse pancreas, Rat kidney; Cellular Location: Membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.98 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Meprin-α (Mep1A) enhances TNF-α secretion by mast cells and aggravates abdominal aortic aneurysms.

Gao R, Liu D, Guo W, Ge W, Fan T, Li B, Gao P, Liu B, Zheng Y, Wang J
British journal of pharmacology 2020 Jun;177(12):2872-2885

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using MEP1A Polyclonal antibody (Product # PA5-96943) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MEP1A in extracts of various cell lines using MEP1A Polyclonal Antibody (Product # PA5-96943) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.