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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- TA342215 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Anti-Sema3f Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Anti-Sema3f Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SEMA3F
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB Suggested Anti-Sema3f Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB Suggested Anti-SEMA3F Antibody ; Positive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sem
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Application: IP; Species+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ug; Primary antibody dilution: 1:1000; Secondary antibody:
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Sample Type :; Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution :; 1:1000 Secondary Antibody :; Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution :; 1:500 Color/Signal Descriptions :; Gene Name :; SEMA3F
- Validation comment
- IHC
