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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB3237 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Semaphorin 3F Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects mouse Semaphorin 3F in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Semaphorin 3A, 3B, 3C, 3E, 4C, 4D, 4G, recombinant human Semaphorin 3D or 3F is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O88632- Isotype
- IgG
- Antibody clone number
- 741307
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Semaphorin 3F in Mouse Brain. Semaphorin 3F was detected in immersion fixed frozen sections of embryonic mouse brain (13 d.p.c.) using Rat Anti-Mouse Semaphorin 3F Monoclonal Antibody (Catalog # MAB3237) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
