MAB6617

antibody from Novus Biologicals

Targeting: STX12 STX13, STX14

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6617

Provider

Novus Biologicals

Product name

Mouse Monoclonal Syntaxin 12 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Syntaxin 12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Syntaxin 1A, 1B1, 1B2, 5, 6, 7, 8, or 16 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Syntaxin 12 by Western Blot. Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line, LNCaP human prostate cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Syntaxin 12 Monoclonal Antibody (Catalog # MAB6617) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Syntaxin 12 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.