Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-118159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of PGK2 in Lane A: HepG2 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-118159) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 45 kDa, Observed band size: 45 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PGK2 Polyclonal Antibody (Product # PA5-118159) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PGK2 in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118159) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 45 kDa , Observed band size : 45 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGK2 Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with PGK2 Polyclonal Antibody (Product # PA5-118159) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PGK2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa. Observed band size: 45 kDa.