MA5-31848

antibody from Invitrogen Antibodies

Targeting: PKHD1 ARPKD, FCYT, FPC, TIGM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31848

Provider

Invitrogen Antibodies

Product name

PKHD1 Monoclonal Antibody (8G12A1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

8G12A1

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PKHD1 in mouse PKHD1 recombinant protein. Sample was incubated with PKHD1 monoclonal antibody (Product # MA5-31848).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of PKHD1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PKHD1 monoclonal antibody (Product # MA5-31848) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of PKHD1 in A431 cells (green). Sample was incubated with PKHD1 monoclonal antibody (Product # MA5-31848) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PKHD1 in paraffin-embedded esophageal cancer tissue. Sample was incubated with PKHD1 monoclonal antibody (Product # MA5-31848) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of PKHD1 in HeLa cells (green). Sample was incubated with PKHD1 monoclonal antibody (Product # MA5-31848) using a dilution of 1:200-1:400 followed by negative control (red).