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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31593 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji, HeLa, HepG2.
- Concentration
- 0.76 mg/mL
Submitted references mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
Gebhardt A, Habjan M, Benda C, Meiler A, Haas DA, Hein MY, Mann A, Mann M, Habermann B, Pichlmair A
Nature communications 2015 Sep 18;6:8192
Nature communications 2015 Sep 18;6:8192
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ARS2 Polyclonal Antibody detects ARS2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. ARS2 Polyclonal Antibody (Product # PA5-31593) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ARS2 in paraformaldehyde-fixed A431 cells using an ARS2 polyclonal antibody (Product # PA5-31593) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2 (Product # PA5-31593) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. ( a ) GFP-tagged NCBP3, NCBP2 or control protein (ctrl) stably expressed in HeLa cells were precipitated and associated proteins were analysed by LC-MS/MS. Volcano plots show the average degrees of enrichment by NCBP3 or NCBP2 over ctrl (ratio of LFQ protein intensities; x axis) and P values ( t -test; y axis) for each protein. Bait proteins (red letters), proteins of the exon junction complex (EJC, turquoise dots) or the TREX complex (purple dots), CBC (magenta dots), NELF (olive dots), importin (green dots) and NCBP3, SRRT and PHAX (red dots) are highlighted. Significantly enriched proteins are separated from the background by a hyperbolic curve. Four independent affinity purifications were performed for each bait. ( b ) Schematic illustration of AP-MS analysis in a focusing on proteins involved in RNA biogenesis. ( c ) Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. ( d ) Binding of NCBP1 to NCBP3 in E. coli . Western blot after His-precipitation using RNAse-treated lysates from bacteria co-expressing recombinant SII-tagged NCBP1 and full-length MBP/His-tagged NCBP3 or MBP/His as control. ( e ) NCBP2 and NCBP3 are required for NCBP1 association to m 7 GTP. Lysates of HeLa cells treated twice with siRNA against NCBP2, NCBP3 or both were used for m 7 GTP-affinity purification (m 7 GTP-AP), followed by western blot analysis. The m 7 GTP-b
