Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009815-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009815-M03, RRID:AB_539132
- Product name
- Mouse Monoclonal GIT2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GIT2 - G protein-coupled receptor kinase interactor 2
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GIT2 Antibody (M1) [H00009815-M03] - Analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody (M03), clone M1. Lane 1: GIT2 transfected lysate (Predicted MW: 52.6 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GIT2 Antibody (M1) [H00009815-M03] - Analysis of GIT2 transfected lysate using anti-GIT2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GIT2 rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GIT2 Antibody (M1) [H00009815-M03] - Detection limit for recombinant GST tagged GIT2 is 1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GIT2 Antibody (M1) [H00009815-M03] - Analysis of monoclonal antibody to GIT2 on HeLa cell. Antibody concentration 10 ug/ml