F54326

antibody from NSJ Bioreagents

Targeting: MYL2 CMH10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54326

Provider

NSJ Bioreagents

Product name

Myosin Light Chain 2 Antibody / MLC2 / MYL2

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 µl, 0.2 µl

Storage

Aliquot the Myosin Light Chain 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human heart, 2) human skeletal muscle, 3) mouse heart and 4) rat heart lysate with Myosin Light Chain 2 antibody. Predicted molecular weight ~19 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human skeletal muscle tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human myocardium tissue with Myosin Light Chain 2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Myosin Light Chain 2 antibody; Blue=isotype control, Green= Myosin Light Chain 2 antibody.