Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-59483-100ul - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Aspartate Aminotransferase Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Lane 1: 293T cell lysates, Lane 2: GOT1 Transfected 293T cell lysates
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).