H00002805-D01

antibody from Abnova Corporation

Targeting: GOT1 AST1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002805-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002805-D01, RRID:AB_10718774

Product name

GOT1 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human GOT1 protein.

Antigen sequence

MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVN
LGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHE
YLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQ
SLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPT
WENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFL
NDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIA
SVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFV
SEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPES
ILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPE
LFEEWTGNVKTMADRILTMRSELRARLEALKTPGT
WNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPS
GRINVSGLTTKNLDYVATSIHEAVTKIQ


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GOT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of GOT1 expression in mouse testis.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GOT1 expression in transfected 293T cell line (H00002805-T01) by GOT1 MaxPab polyclonal antibody.Lane 1: GOT1 transfected lysate(46.2 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GOT1 MaxPab rabbit polyclonal antibody. Western Blot analysis of GOT1 expression in K-562.

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of GOT1 transfected lysate using anti-GOT1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GOT1 purified MaxPab mouse polyclonal antibody (B01P) (H00002805-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of purified MaxPab rabbit antibody to GOT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol