60317-1-IG

antibody from Invitrogen Antibodies

Targeting: GOT1 AST1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

60317-1-IG

Provider

Invitrogen Antibodies

Product name

GOT1 Monoclonal Antibody (5A12E10)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MAPPSVFAE VPQAQPVLVF KLTADFREDP DPRKVNLGVG AYRTDDCHPW VLPVVKKVEQ KIANDNSLNH EYLPILGLAE FRSCASRLAL GDDSPALKEK RVGGVQSLGG TGALRIGADF LARWYNGTNN KNTPVYVSSP TWENHNAVFS AAGFKDIRSY RYWDAEKRGL DLQGFLNDLE NAPEFSIVVL HACAHNPTGI DPTPEQWKQI ASVMKHRFLF PFFDSAYQGF ASGNLERDAW AIRYFVSEGF EFFCAQSFSK NFGLYNERVG NLTVVGKEPE SILQVLSQME KIVRITWSNP PAQGARIVAS TLSNPELFEE WTGNVKTMAD RILTMRSELR ARLEALKTPG TWNHITDQIG MFSFTGLNPK QVEYLVNEKH IYLLPSGRIN VSGLTTKNLD YVATSIHEAV TKIQ (1-413 aa encoded by BC000498)

Reactivity

Human, Mouse, Rat, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

5A12E10

Vial size

150 µL

Concentration

0.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 60317-1-IG ( GOT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 60317-1-IG (GOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 60317-1-IG (GOT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 60317-1-IG ( GOT1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 60317-1-IG ( GOT1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.20ug GOT1 antibody (60317-1-IG, red) and control antibody (blue). Fixed with 90% MeOH.