10286-1-AP

antibody from Invitrogen Antibodies

Targeting: PLAUR CD87, UPAR, URKR

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10286-1-AP

Provider

Invitrogen Antibodies

Product name

uPAR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGHPPLLPL LLLLHTCVPA SWGLRCMQCK TNGDCRVEEC ALGQDLCRTT IVRLWEEGEE LELVEKSCTH SEKTNRTLSY RTGLKITSLT EVVCGLDLCN QGNSGRAVTY SRSRYLECIS CGSSDMSCER GRHQSLQ (1-136 aa encoded by BC002788)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A2780 cells were subjected to SDS PAGE followed by western blot with 10286-1-AP (uPAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 U-937 cells were stained with 0.2ug uPAR antibody (10286-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-uPAR, PLAUR (IP: 10286-1-AP, 4 µg; Detection: 10286-1-AP 1:300) with RAW 264.7 cells lysate 4000 µg.