PA5-118248

antibody from Invitrogen Antibodies

Targeting: RPL29 HIP, HUMRPL29, L29, RPL29P10

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118248

Provider

Invitrogen Antibodies

Product name

RPL29 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using RPL29 Polyclonal Antibody (Product # PA5-118248) at 1:500 dilution. Lane A: PC-3 Whole Cell Lysate, Lane B: U87-MG Whole Cell Lysate, Lane C: SW480 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of RPL29 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with RPL29 Polyclonal Antibody (Product # PA5-118248, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human RPL29 in human brain with RPL29 Polyclonal Antibody (Product # PA5-118248, 1:10,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human RPL29 in human liver with RPL29 Polyclonal Antibody (Product # PA5-118248, 1:10,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RPL29 Immunoprecipitation using: Lane A: 0.5 mg PC-3 Whole Cell Lysate 4 µL with RPL29 Polyclonal Antibody (Product # PA5-118248) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: RPL29 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 22 kDa.