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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [6]
- Immunocytochemistry [5]
- Immunohistochemistry [2]
- Other assay [2]
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- Product number
- PA5-21712 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse heart.
- Concentration
- 1.56 mg/mL
Submitted references WNT11, a new gene associated with early onset osteoporosis, is required for osteoblastogenesis.
16p11.2 transcription factor MAZ is a dosage-sensitive regulator of genitourinary development.
Role of Noncanonical Wnt Signaling Pathway in Human Aortic Valve Calcification.
Caetano da Silva C, Edouard T, Fradin M, Aubert-Mucca M, Ricquebourg M, Raman R, Salles JP, Charon V, Guggenbuhl P, Muller M, Cohen-Solal M, Collet C
Human molecular genetics 2022 May 19;31(10):1622-1634
Human molecular genetics 2022 May 19;31(10):1622-1634
16p11.2 transcription factor MAZ is a dosage-sensitive regulator of genitourinary development.
Haller M, Au J, O'Neill M, Lamb DJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 20;115(8):E1849-E1858
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 20;115(8):E1849-E1858
Role of Noncanonical Wnt Signaling Pathway in Human Aortic Valve Calcification.
Albanese I, Yu B, Al-Kindi H, Barratt B, Ott L, Al-Refai M, de Varennes B, Shum-Tim D, Cerruti M, Gourgas O, Rhéaume E, Tardif JC, Schwertani A
Arteriosclerosis, thrombosis, and vascular biology 2017 Mar;37(3):543-552
Arteriosclerosis, thrombosis, and vascular biology 2017 Mar;37(3):543-552
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WNT11 using A) 30 µg 293T whole cell extract (B) 30 µg A431 whole cell extract (C) 30 µg HeLa whole cell extract and D) 30 µg HepG2 whole cell extract. Samples were loaded onto a 12% SDS-PAGE gel and probed with a WNT11 polyclonal antibody (Product # PA5-21712) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WNT11 using 50 µg mouse heart extract. Samples were loaded onto a 10% SDS-PAGE gel and probed with a WNT11 polyclonal antibody (Product # PA5-21712) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using WNT11 Polyclonal Antibody (Product # PA5-21712). Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Caco-2 (Lane 1), U2OS (Lane 2), HeLa (Lane 3), HT-29 (Lane 4), HEK-293 (Lane 5), MCF7 (Lane 6), PC-3 (Lane 7) and A-431 (Lane 8). The blot was probed with Anti-WNT11 Polyclonal Antibody (Product # PA5-21712, 1µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). A 40 kDa band corresponding to WNT11 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WNT11 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a WNT11 polyclonal antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WNT11 in paraformaldehyde-fixed HeLa cells using a WNT11 polyclonal antibody (Product # PA5-21712) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNT11 Polyclonal Antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11 antibody (Product # PA5-21712) at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNT11 Polyclonal Antibody detects WNT11 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: WNT11 protein stained by WNT11 Polyclonal Antibody (Product # PA5-21712) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11 (Product # PA5-21712) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. WNT11 antibody (Product # PA5-21712) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 WNT11 heterozygous mutant cells with decreased WNT11 mRNA and protein levels as well as proliferation and mineralization. ( A ) Electropherogram showing the 32-bp deletion leading to a frameshift. fs * : frameshift. ( B ) RT-qPCR analysis of WNT11 mRNA expression in mutant versus control U2OS cells. Normalization was to GAPDH level as a housekeeping gene with ratio of one for control U2OS cells. Ctrl: wild-type U2OS cells, Mut: WNT11 mutant cells, FC: fold change. ( C ) Western blot analysis of WNT11 protein expression, confirming the heterozygosity. a-Tubulin was a control. ( D ) Alizarin red staining showed formation of mineralized nodules after osteogenic differentiation treatment with osteogenic media in control and WNT11 mutant cells. ( E ) Proliferation of control and WNT11 mutant cells ( n = 42). Data are mean +- SEM. * P < 0.05, ** P < 0.01, **** P < 0.0001.
