ABIN1381739

antibody from antibodies-online

Targeting: CEACAM16 DFNA4B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1381739

Provider

antibodies-online

Product name

anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 16 (CEACAM16) antibody

Antibody type

Monoclonal

Antigen

genetic immunisation with cDNA encoding human CEACAM16

Description

Protein G

Reactivity

Human

Isotype

IgG

Antibody clone number

SU-9D5

Vial size

100 μg

Storage

short term: 2°C - 8°C, long term: -20°C

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Loss of mammal-specific tectorial membrane component carcinoembryonic antigen cell adhesion molecule 16 (CEACAM16) leads to hearing impairment at low and high frequencies.

Kammerer R, Rüttiger L, Riesenberg R, Schäuble C, Krupar R, Kamp A, Sunami K, Eisenried A, Hennenberg M, Grunert F, Bress A, Battaglia S, Schrewe H, Knipper M, Schneider MR, Zimmermann W
The Journal of biological chemistry 2012 Jun 22;287(26):21584-98

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

SDS-PAGE analysis of purified SU-9D5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified SU-9D5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunohistological staining using SU-9D5 on paraffin sections of cochlea tissue of an adult transgenic mouse with a human CEACAM16 gene (Ceacam16+/+ mouse cochlea). Binding of biotinylated SU-9D5 was detected with horseradish peroxidase-coupled streptavidin and stained with 3-amino-9-ethylcarbazole.

Flow cytometry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

FACS analysis of BOSC23 cells using SU-9D5. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM16 (red curve) or an irrelevant protein (control transfectant). Binding of SU-9D5 was detected with a PE- conjugated secondary antibody. A positive signal was obtained only with CEACAM16 transfected cells.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

BOSC23 cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, CEACAM3, 4, 6, 8 19, or 20. Expression of the constructs was tested with monoclonal antibodies known to recognize the corresponding proteins (data not shown). An irrelevant monoclonal antibody served as a negative control (black curves). For specificity testing, protein G-purified SU-9D5 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM16 transfected cells (red curve).