11287-1-AP

antibody from Proteintech Group

Targeting: EIF3I eIF3-beta, eIF3-p36, EIF3S2, TRIP-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

11287-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#11287-1-AP, RRID:AB_2097068

Product name

EIF3I antibody

Antibody type

Polyclonal

Description

KD/KO validated EIF3I antibody (Cat. #11287-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis.

Roobol A, Roobol J, Smith ME, Carden MJ, Hershey JWB, Willis AE, Smales CM
Metabolic engineering 2020 May;59:98-105

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Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.

Zhang Y, Zhang Y, Xiao Y, Zhong C, Xiao F
Ecotoxicology and environmental safety 2019 Nov 15;183:109465

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EIF3i affects vesicular stomatitis virus growth by interacting with matrix protein.

Pan W, Song D, He W, Lu H, Lan Y, Li H, Gao F, Zhao K
Veterinary microbiology 2017 Dec;212:59-66

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Biological insights into the expression of translation initiation factors from recombinant CHOK1SV cell lines and their relationship to enhanced productivity.

Mead EJ, Masterton RJ, Feary M, Obrezanova O, Zhang L, Young R, Smales CM
The Biochemical journal 2015 Dec 15;472(3):261-73

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The chaperonin CCT interacts with and mediates the correct folding and activity of three subunits of translation initiation factor eIF3: b, i and h.

Roobol A, Roobol J, Carden MJ, Smith ME, Hershey JW, Bastide A, Knight JR, Willis AE, Smales CM
The Biochemical journal 2014 Mar 1;458(2):213-24

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TRIP-1: a regulator of osteoblast function.

Metz-Estrella D, Jonason JH, Sheu TJ, Mroczek-Johnston RM, Puzas JE
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Jul;27(7):1576-84

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11287-1-AP(EIF3I antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using EIF3I antibody 11287-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-EIF3I (IP:11287-1-AP, 3ug; Detection:11287-1-AP 1:1000) with HEK-293 cells lysate 1500ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 11287-1-AP(EIF3I antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue