Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN955091 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-TBC1 Domain Family, Member 13 (TBC1D13) (AA 83-113), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 83-113 amino acids from the Central region of Human TBC1D13
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- AA 83-113,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity.
Ishibashi K, Kanno E, Itoh T, Fukuda M
Genes to cells : devoted to molecular & cellular mechanisms 2009 Jan;14(1):41-52
Genes to cells : devoted to molecular & cellular mechanisms 2009 Jan;14(1):41-52
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using TBC1D13 Antibody (Center) Cat.-No AP54165PU-N in CEM cell line lysates (35μg/lane). This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis using TBC1D13 Antibody (Center) Cat.-No AP54165PU-N in Mouse spleen tissue lysates (35μg/lane). This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of TBC1D13 Antibody (Center) Cat.-No AP54165PU-N with NCI-H460 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin Fixed, Paraffin Embedded Mouse heart tissue stained with TBC1D13 Antibody (Center) Cat.-No AP54165PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TBC1D13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.