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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- NB100-1358 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1358, RRID:AB_2167159
- Product name
- Goat Polyclonal DNA Polymerase lambda Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core), but considering only the peptide that we used as immunogen, the two share the sequence YREP**R** at the C-terminus. Furthermore, the protein structures appear to be very similar in this region.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references DNA polymerase lambda, a novel DNA repair enzyme in human cells.
García-Díaz M, Bebenek K, Sabariegos R, Domínguez O, Rodríguez J, Kirchhoff T, García-Palomero E, Picher AJ, Juárez R, Ruiz JF, Kunkel TA, Blanco L
The Journal of biological chemistry 2002 Apr 12;277(15):13184-91
The Journal of biological chemistry 2002 Apr 12;277(15):13184-91
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DNA Polymerase lambda Antibody [NB100-1358] - Staining of A549 (A) and HepG2 (B) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
