Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB100-81665 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-81665, RRID:AB_1145388
- Product name
- Rabbit Polyclonal DNA Polymerase lambda Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-81665 used for IP at 3 mcg/mg lysate. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664, which recognizes an upstream epitope.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81665 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664. For blotting immunoprecipitated Pol Lambda, NB100-81665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Pol Lambda used at a dilution of 1:1,000 (1 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - FFPE section of human prostate nodular hypertrophy. NB100-81665 used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DNA Polymerase lambda Antibody [NB100-81665] - Detection of mouse Pol Lambda by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB