MAB323-100

antibody from R&D Systems

Targeting: FGF8 AIGF

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB323-100

Provider

R&D Systems

Product name

Human/Mouse FGF-8 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human and mouse FGF-8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) FGF-5, rhFGF-7, and rhFGF-9 is observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

NP_001159834


Isotype

IgG

Antibody clone number

47109

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Specificity Protein 7 Is Required for Proliferation and Differentiation of Ameloblasts and Odontoblasts.

Bae JM, Clarke JC, Rashid H, Adhami MD, McCullough K, Scott JS, Chen H, Sinha KM, de Crombrugghe B, Javed A
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Jun;33(6):1126-1140

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Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: EFFECTS ON EMBRYO SURVIVAL.

Fresco VM, Kern CB, Mohammadi M, Twal WO
The Journal of biological chemistry 2016 Sep 2;291(36):18730-9

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Local apoptosis modulates early mammalian brain development through the elimination of morphogen-producing cells.

Nonomura K, Yamaguchi Y, Hamachi M, Koike M, Uchiyama Y, Nakazato K, Mochizuki A, Sakaue-Sawano A, Miyawaki A, Yoshida H, Kuida K, Miura M
Developmental cell 2013 Dec 23;27(6):621-34

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Cell aggregation-induced FGF8 elevation is essential for P19 cell neural differentiation.

Wang C, Xia C, Bian W, Liu L, Lin W, Chen YG, Ang SL, Jing N
Molecular biology of the cell 2006 Jul;17(7):3075-84

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Regulation of avian cardiogenesis by Fgf8 signaling.

Alsan BH, Schultheiss TM
Development (Cambridge, England) 2002 Apr;129(8):1935-43

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FGF-8 in Human Prostate. FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by FGF-8 and Neutralization by Human and Mouse FGF-8 Antibody. Recombinant Mouse FGF-8 c Isoform (Catalog # 424-FC) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse FGF-8 c Isoform (125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse FGF-8 Monoclonal Antibody (Catalog # MAB323). The ND₅₀ is typically 0.25-0.75 µg/mL in the presence of heparin (0.1 µg/mL).