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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-423-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse FGF-8b Isoform Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse FGF-8b Isoform in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) FGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7 and rhFGF-9 is observed. It does, however, cross-react with recombinant mouse FGF-8c in neutralizing bioassay.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_006110- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fgf20 is required for sensory epithelial specification in the developing cochlea.
Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx.
FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
Hayashi T, Ray CA, Bermingham-McDonogh O
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jun 4;28(23):5991-9
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Jun 4;28(23):5991-9
Cardiac arterial pole alignment is sensitive to FGF8 signaling in the pharynx.
Hutson MR, Zhang P, Stadt HA, Sato AK, Li YX, Burch J, Creazzo TL, Kirby ML
Developmental biology 2006 Jul 15;295(2):486-97
Developmental biology 2006 Jul 15;295(2):486-97
FGF8 is required for cell survival at distinct stages of nephrogenesis and for regulation of gene expression in nascent nephrons.
Grieshammer U, Cebrián C, Ilagan R, Meyers E, Herzlinger D, Martin GR
Development (Cambridge, England) 2005 Sep;132(17):3847-57
Development (Cambridge, England) 2005 Sep;132(17):3847-57
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- FGF-8 in Human Prostate. FGF-8 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Mouse FGF-8 b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by FGF-8 and Neutralization by Mouse FGF-8 Antibody. Recombinant Mouse FGF-8b Isoform (Catalog # 423-F8) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse FGF-8b Isoform (60 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse FGF-8b Isoform Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-423-NA). The ND50 is typically 0.4-2.4 µg/mL in the presence of heparin (1 µg/mL).
