Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- ARG54961 - Provider product page
- Provider
- Arigo
- Product name
- anti-cGAS antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 266-295 (Center) of Human CF150.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of HT-29, THP-1 cell line, human cerebellum tissue lysate (from left to right) stained with ARG54961 anti-cGAS antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of MCF-7, HT-29, THP-1 cell line, human spleen, human cerebellum tissue lysate(from left to right) stained with ARG54961 anti-cGAS antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of THP-1 cell line stained with ARG54961 anti-cGAS antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: MDA-MB231 cells stained with ARG54961 anti-cGAS antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: A549 cells stained with ARG54961 anti-cGAS antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.