Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
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- Product number
- NBP1-86761 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86761, RRID:AB_11040051
- Product name
- Rabbit Polyclonal cGAS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human cGAS antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
Listeria monocytogenes induces IFNβ expression through an IFI16-, cGAS- and STING-dependent pathway.
Berg RK, Rahbek SH, Kofod-Olsen E, Holm CK, Melchjorsen J, Jensen DG, Hansen AL, Jørgensen LB, Ostergaard L, Tolstrup M, Larsen CS, Paludan SR, Jakobsen MR, Mogensen TH
PloS one 2014;9(1):e84513
PloS one 2014;9(1):e84513
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
Schoggins JW, MacDuff DA, Imanaka N, Gainey MD, Shrestha B, Eitson JL, Mar KB, Richardson RB, Ratushny AV, Litvak V, Dabelic R, Manicassamy B, Aitchison JD, Aderem A, Elliott RM, García-Sastre A, Racaniello V, Snijder EJ, Yokoyama WM, Diamond MS, Virgin HW, Rice CM
Nature 2014 Jan 30;505(7485):691-5
Nature 2014 Jan 30;505(7485):691-5
Listeria monocytogenes induces IFNβ expression through an IFI16-, cGAS- and STING-dependent pathway.
Hansen K, Prabakaran T, Laustsen A, Jørgensen SE, Rahbæk SH, Jensen SB, Nielsen R, Leber JH, Decker T, Horan KA, Jakobsen MR, Paludan SR
The EMBO journal 2014 Aug 1;33(15):1654-66
The EMBO journal 2014 Aug 1;33(15):1654-66
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: cGAS Antibody [NBP1-86761] - Simple Western lane view shows a specific band for MB21D1 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: cGAS Antibody [NBP1-86761] - Electropherogram image(s) of corresponding Simple Western lane view. MB21D1 antibody was used at 1:30 dilution on RT-4 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: cGAS Antibody [NBP1-86761] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-MB21D1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages and a subset of leukocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in lymphoid cells in lamina propria.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human placenta shows moderate cytoplasmic positivity in Hoffbauer cells and placental trophoblast.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human tonsil shows moderate cytoplasmic positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human tonsil shows moderate to strong granular cytoplasmic positivity in non-germinal center and germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: cGAS Antibody [NBP1-86761] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells