MA5-34709

antibody from Invitrogen Antibodies

Targeting: EEF1A1 EE1A1, EEF1A, EF1A, LENG7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34709

Provider

Invitrogen Antibodies

Product name

EEF1A1 Recombinant Rabbit Monoclonal Antibody (JB44-13)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Rat brain tissue, mouse skeletal muscle tissue lysate, rat skin tissue lysate, Daudi, mouse cerebellum tissue lysate, HepG2, HUVEC, SH-SY-5Y, rat skeletal muscle tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse smooth muscle tissue, THP-1.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB44-13

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of eEF1A1 in Lane 1: Rat brain tissue, Lane 2: Mouse skeletal muscle, Lane 3: Mouse cerebellum, Lane 4: Rat skin, Lane 5: Daudi . Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of eEF1A1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of eEF1A1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of eEF1A1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eEF1A1 in paraffin-embedded rat skeletal muscle tissue. Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eEF1A1 in paraffin-embedded mouse smooth muscle tissue. Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eEF1A1 in paraffin-embedded human tonsil tissue. Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eEF1A1 in paraffin-embedded human liver cancer tissue. Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of eEF1A1 in paraffin-embedded human kidney tissue. Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of eEF1A1 in THP-1 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.