H00023028-M04

antibody from Abnova Corporation

Targeting: KDM1A AOF2, BHC110, KDM1, KIAA0601, LSD1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023028-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023028-M04, RRID:AB_565904

Product name

AOF2 monoclonal antibody (M04), clone 2E7

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant AOF2.

Antigen sequence

ADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIP
GAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREA
GRIADQFLGAMYTLPRQATPGVPAQQSPS


Isotype

IgG

Antibody clone number

2E7

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Characterization of histone lysine-specific demethylase in relation to thyroid hormone-regulated anuran metamorphosis.

Chen W, Obara M, Ishida Y, Suzuki K, Yoshizato K
Development, growth & differentiation 2007 May;49(4):325-34

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AOF2 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to AOF2 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to AOF2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol