Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-695 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-695, RRID:AB_10000851
- Product name
- Rabbit Polyclonal VDAC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This reacts with all forms of VDAC, including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms).
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Overexpression of lncRNA EPB41L4A-AS1 Induces Metabolic Reprogramming in Trophoblast Cells and Placenta Tissue of Miscarriage.
Phosphorylation of the BNIP3 C-Terminus Inhibits Mitochondrial Damage and Cell Death without Blocking Autophagy.
Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting.
Effect of valproic acid on mitochondrial epigenetics.
Zhu Y, Liu Q, Liao M, Diao L, Wu T, Liao W, Wang Z, Li B, Zhang S, Wang S, Xie W, Jiang Y, Xu N, Zeng Y, Yang BB, Zhang Y
Molecular therapy. Nucleic acids 2019 Dec 6;18:518-532
Molecular therapy. Nucleic acids 2019 Dec 6;18:518-532
Phosphorylation of the BNIP3 C-Terminus Inhibits Mitochondrial Damage and Cell Death without Blocking Autophagy.
Liu KE, Frazier WA
PloS one 2015;10(6):e0129667
PloS one 2015;10(6):e0129667
Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting.
Su YC, Chiu HW, Hung JC, Hong JR
Apoptosis : an international journal on programmed cell death 2014 Oct;19(10):1457-70
Apoptosis : an international journal on programmed cell death 2014 Oct;19(10):1457-70
Effect of valproic acid on mitochondrial epigenetics.
Chen H, Dzitoyeva S, Manev H
European journal of pharmacology 2012 Sep 5;690(1-3):51-9
European journal of pharmacology 2012 Sep 5;690(1-3):51-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY licence.
