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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA324500 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal SIX5 Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal SIX5 Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SIX5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SIX5 Antibody (Center) (Cat. #TA324500) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of Hela cell stained with SIX5 Antibody (Center)(Cat#TA324500).Hela cells were incubated with SIX5 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . SIX5 immunoreactivity is localized to Cytoplasm significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- SIX5 Antibody (Center) (Cat. #TA324500)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
