Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN954812 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Six5 (AA 264-294), (Middle Region) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 264-294 amino acids from the Central region of Human SIX5. Genename: SIX5
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 264-294,Middle Region
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals.
Association of genetic variants with hemorrhagic stroke in Japanese individuals.
Transcription factor SIX5 is mutated in patients with branchio-oto-renal syndrome.
Identification of transcriptional targets for Six5: implication for the pathogenesis of myotonic dystrophy type 1.
Oguri M, Kato K, Yokoi K, Yoshida T, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y
American journal of hypertension 2010 Jan;23(1):70-7
American journal of hypertension 2010 Jan;23(1):70-7
Association of genetic variants with hemorrhagic stroke in Japanese individuals.
Yoshida T, Kato K, Yokoi K, Oguri M, Watanabe S, Metoki N, Yoshida H, Satoh K, Aoyagi Y, Nozawa Y, Yamada Y
International journal of molecular medicine 2010 Apr;25(4):649-56
International journal of molecular medicine 2010 Apr;25(4):649-56
Transcription factor SIX5 is mutated in patients with branchio-oto-renal syndrome.
Hoskins BE, Cramer CH, Silvius D, Zou D, Raymond RM, Orten DJ, Kimberling WJ, Smith RJ, Weil D, Petit C, Otto EA, Xu PX, Hildebrandt F
American journal of human genetics 2007 Apr;80(4):800-4
American journal of human genetics 2007 Apr;80(4):800-4
Identification of transcriptional targets for Six5: implication for the pathogenesis of myotonic dystrophy type 1.
Sato S, Nakamura M, Cho DH, Tapscott SJ, Ozaki H, Kawakami K
Human molecular genetics 2002 May 1;11(9):1045-58
Human molecular genetics 2002 May 1;11(9):1045-58
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of SIX5 Antibody Cat.-No AP53922PU-N in MDA-MB231 cell line lysates (35ug/lane). This demonstrates the SIX5 antibody detected the SIX5 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human liver tissue stained with SIX5 Antibody Cat.-No AP53922PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SIX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.