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antibody from Invitrogen Antibodies
Targeting: NOLC1
KIAA0035, NOPP130, NOPP140, P130, Srp40
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-96472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOLC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKPAAAPKQP VGGGQKLLTR KADSSSSEEE SSSSEEEKTK KMVATTKPKA TAKAALSLPA KQAPQGSRDS SSDSDSSSSE EEEEKTSKSA VKKKPQKVAG GAAPSKPASA KKGKAESSNS SSSDDSSEEE EEKLKGKGSP RPQAPKANGT SALTAQNGKA AKNSEEEEEE KKKAAVVVSK SGSLKKRKQN EAAKEAETPQ AKKIKLQTPN TFPKRKKGEK RASSPFRRVR EEEIEVDSRV ADNSFDAKRG AAGDWGERAN QVLKFTKGKS FRHEKTKKKR GSYRGGSISV QVNSIKFDSE; Positive Samples: A-549, HeLa, HepG2, MCF7; Cellular Location: Cytoplasm, Nucleus, nucleolus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NOLC1 in extracts of various cell lines using NOLC1 Polyclonal Antibody (Product # PA5-96472) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of NOLC1 was performed in U-2 OS cells using NOLC1 Polyclonal Antibody (Product # PA5-96472) at a dilution of 1:100. Blue: DAPI for nuclear staining.
