11815-1-AP

antibody from Invitrogen Antibodies

Targeting: NOLC1 KIAA0035, NOPP130, NOPP140, P130, Srp40

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11815-1-AP

Provider

Invitrogen Antibodies

Product name

NOLC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TKPPPAKKA AESSSDSSDS DSSEDDEAPS KPAGTTKNSS NKPAVTTKSP AVKPAAAPKQ PVGGGQKLLT RKADSSSSEE ESSSSEEEKT KKMVATTKPK ATAKAALSLP AKQAPQGSRD SSSDSDSSSS EEEEEKTSKS AVKKKPQKVA GGAAPSKPAS AKKGKAESSN SSSSDDSSEE EEEKLKGKGS PRPQAPKANG TSALTAQNGK AAKNSEEEEE EKKKAAVVVS KSGSLKKRKQ NEAAKEAETP QAKKIKLQTP NTFPKRKKGE KRASSPFRRV REEEIEVDSR VADNSFDAKR GAAGDWGERA NQVLKFTKGK SFRHEKTKKK RGSYRGGSIS VQVNSIKFDS E (69-418 aa encoded by BC006769)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP ( NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 11815-1-AP (NOLC1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NOLC1 antibody 11815-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 11815-1-AP (NOLC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using NOLC1 antibody 11815-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 11815-1-AP (NOLC1 antibody) at dilution of 1:50 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-NOLC1 (IP:11815-1-AP, 4ug; Detection:11815-1-AP 1:500) with HeLa cells lysate 2000ug.