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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- NBP1-22981 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-22981, RRID:AB_1726360
- Product name
- Rabbit Polyclonal NOLC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NOLC1 Antibody [NBP1-22981] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from K562 cells. Affinity purified rabbit anti-NOPP140 antibody used for IP at 3 mcg/mg lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NOLC1 Antibody [NBP1-22981] - Detection of human NOPP140 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K-562 cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody NBP1-22981 used for IP at 3 ug/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody NBP1-22982, which recognizes a downstream epitope. For blotting immunoprecipitated NOPP140, NBP1-22981 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NOPP140 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
