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LearnH00220047-M01
antibody from Abnova Corporation
Targeting: CCDC83
CT148, FLJ42119, KP-CoT-23, MGC34732
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00220047-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00220047-M01, RRID:AB_1137308
- Product name
- MGC34732 monoclonal antibody (M01), clone 3E3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant MGC34732.
- Antigen sequence
SSDESTILHLSHENSIEDLQYVKIDKEENSGTEFG
DTDMKYLLYEDEKDFKDYVNLGPLGVKLMSVESKK
MPIHFQEKEIPVKLYKDVRSPESHITYKMMKSFL- Isotype
- IgG
- Antibody clone number
- 3E3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CCDC83 expression in transfected 293T cell line by MGC34732 monoclonal antibody (M01), clone 3E3.Lane 1: CCDC83 transfected lysate (Predicted MW: 48.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CCDC83 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CCDC83 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
