Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 16818-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EED Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSEREVSTA PAGTDMPAAK KQKLSSDENS NPDLSGDEND DAVSIESGTN TERPDTPTNT PNAPGRKSWG KGKWKSKKCK YSFKCVNSLK EDHNQPLFGV QFNWHSKEGD PLVFATVGSN RVTLYECHSQ GEIRLLQSYV DADADENFYT CAWTYDSNTS HPLLAVAGSR GIIRIINPIT MQCIKHYVGH GNAINELKFH PRDPNLLLSV SKDHALRLWN IQTDTLVAIF GGVEGHRDEV LSADYDLLGE KIMSCGMDHS LKLWRINSKR MMNAIKESYD YNPNKTNRPF ISQKIHFPDF STRDIHRNYV DCVRWLGDLI LSKSGRAILH SHQQCMRDPV SPNLRQHLSC ENAIVCWKPG KMEDDIDKIK PSESNVTILG RFDYSQCDIW YMRFSMDFWQ KMLALGNQVG KLYVWDLEVE DPHKAKCTTL THHKCGAAIR QTSFSRDSSI LIAVCDDASI WRWDRLR (1-466 aa encoded by BC068995)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 16818-1-AP (EED Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formalin) fixed HepG2 cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16818-1-AP (EED antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP ( EED Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 16818-1-AP ( EED Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EED (IP:16818-1-AP, 4ug; Detection:16818-1-AP 1:500) with PC-3 cells lysate 3200ug.