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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-32232 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal VAMP-7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Cytotoxic Granule Exocytosis From Human Cytotoxic T Lymphocytes Is Mediated by VAMP7.
Chitirala P, Ravichandran K, Galgano D, Sleiman M, Krause E, Bryceson YT, Rettig J
Frontiers in immunology 2019;10:1855
Frontiers in immunology 2019;10:1855
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: VAMP-7 Antibody [NBP2-32232] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-VAMP7 antibody NBP2-32232 used for IP at 6 ug per reaction. VAMP7 was also immunoprecipitated by rabbit anti-VAMP7 antibody BL16273. For blotting immunoprecipitated VAMP7, NBP2-32232 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
