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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- H00005536-B01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005536-B01, RRID:AB_982820
- Product name
- PPP5C MaxPab mouse polyclonal antibody (B01)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human PPP5C protein.
- Antigen sequence
MAMAEGERTECAEPPRDEPPADGALKRAEELKTQA
NDYFKAKDYENAIKFYSQAIELNPSNAIYYGNRSL
AYLRTECYGYALGDATRAIELDKKYIKGYYRRAAS
NMALGKFRAALRDYETVVKVKPHDKDAKMKYQECN
KIVKQKAFERAIAGDEHKRSVVDSLDIESMTIEDE
YSGPKLEDGKVTISFMKELMQWYKDQKKLHRKCAY
QILVQVKEVLSKLSTLVETTLKETEKITVCGDTHG
QFYDLLNIFELNGLPSETNPYIFNGDFVDRGSFSV
EVILTLFGFKLLYPDHFHLLRGNHETDNMNQIYGF
EGEVKAKYTAQMYELFSEVFEWLPLAQCINGKVLI
MHGGLFSEDGVTLDDIRKIERNRQPPDSGPMCDLL
WSDPQPQNGRSISKRGVSCQFGPDVTKAFLEENNL
DYIIRSHEVKAEGYEVAHGGRCVTVFSAPNYCDQM
GNKASYIHLQGSDLRPQFHQFTAVPHPNVKPMAYA
NTLLQLGMM- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Greatwall-phosphorylated Endosulfine is both an inhibitor and a substrate of PP2A-B55 heterotrimers.
Williams BC, Filter JJ, Blake-Hodek KA, Wadzinski BE, Fuda NJ, Shalloway D, Goldberg ML
eLife 2014 Mar 11;3:e01695
eLife 2014 Mar 11;3:e01695
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PPP5C expression in transfected 293T cell line (H00005536-T01) by PPP5C MaxPab polyclonal antibody.Lane 1: PPP5C transfected lysate(54.89 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PPP5C MaxPab polyclonal antibody. Western Blot analysis of PPP5C expression in human liver.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to PPP5C on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
