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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NBP1-47927 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-47927, RRID:AB_10010919
- Product name
- Mouse Monoclonal PP5 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified. This antibody is specific for Homo sapiens protein phosphatase 5, catalytic subunit (PPP5C).
- Reactivity
- Human, Rat, Canine, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PP5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PP5 Antibody (2E12) [NBP1-47927] Analysis of extracts (35ug) from 9 different cell lines by using anti-PP5 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human endometrium tissue using anti-PP5 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human Ovary tissue using anti-PP5 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PP5 Antibody (2E12) [NBP1-47927] - Staining of paraffin-embedded Human pancreas tissue using anti-PP5 mouse monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PP5 Antibody (2E12) [NBP1-47927] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PP5 antibody, and then analyzed by flow cytometry.
