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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-50021 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-50021, RRID:AB_10012211
- Product name
- Rabbit Polyclonal CCDC8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Identifying biological pathways that underlie primordial short stature using network analysis.
Differential effects on p53-mediated cell cycle arrest vs. apoptosis by p90.
Hanson D, Stevens A, Murray PG, Black GC, Clayton PE
Journal of molecular endocrinology 2014 Jun;52(3):333-44
Journal of molecular endocrinology 2014 Jun;52(3):333-44
Differential effects on p53-mediated cell cycle arrest vs. apoptosis by p90.
Dai C, Tang Y, Jung SY, Qin J, Aaronson SA, Gu W
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 22;108(47):18937-42
Proceedings of the National Academy of Sciences of the United States of America 2011 Nov 22;108(47):18937-42
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: CCDC8 Antibody [NBP1-50021] - Detection of Human CCDC8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and K562 (K; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CCDC8 antibody used for WB at 0.04 mcg/ml (A) and 0.1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. CCDC8 was also immunoprecipitated by rabbit anti-CCDC8 antibody which recognizes a downstream epitope. For blotting immunoprecipitated CCDC8, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
