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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4262 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4262, RRID:AB_1571364
- Product name
- ARL3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ARL3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Assignment of the human ADP-ribosylation factor-like 3 (ARL3) gene to chromosome 10 band q23.3 by radiation hybrid mapping.
Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.
ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues.
Kim HS
Cytogenetics and cell genetics 1998;83(3-4):246
Cytogenetics and cell genetics 1998;83(3-4):246
Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence.
Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, Kirkness EF, Weinstock KG, Gocayne JD, White O
Nature 1995 Sep 28;377(6547 Suppl):3-174
Nature 1995 Sep 28;377(6547 Suppl):3-174
ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues.
Cavenagh MM, Breiner M, Schurmann A, Rosenwald AG, Terui T, Zhang C, Randazzo PA, Adams M, Joost HG, Kahn RA
The Journal of biological chemistry 1994 Jul 22;269(29):18937-42
The Journal of biological chemistry 1994 Jul 22;269(29):18937-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ARL3 (arrow) using rabbit ARL3 polyclonal antibody (Cat # PAB4262). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ARL3 gene (Lane 2) (Origene Technologies).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ARL3 polyclonal antibody (Cat # PAB4262) in A2058 cell line lysate (35 ug/lane). ARL3 (arrow) was detected using the purified ARL3 polyclonal antibody (Cat # PAB4262).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARL3 polyclonal antibody (Cat # PAB4262) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
