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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3584 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3584, RRID:AB_1575211
- Product name
- GJB3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GJB3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mutation analysis of Connexin 31 (GJB3) in sporadic non-syndromic hearing impairment.
Expression of a connexin31 mutation causing erythrokeratodermia variabilis is lethal for HeLa cells.
Defective trafficking and cell death is characteristic of skin disease-associated connexin 31 mutations.
Mhatre AN, Weld E, Lalwani AK
Clinical genetics 2003 Feb;63(2):154-9
Clinical genetics 2003 Feb;63(2):154-9
Expression of a connexin31 mutation causing erythrokeratodermia variabilis is lethal for HeLa cells.
Diestel S, Richard G, Döring B, Traub O
Biochemical and biophysical research communications 2002 Aug 23;296(3):721-8
Biochemical and biophysical research communications 2002 Aug 23;296(3):721-8
Defective trafficking and cell death is characteristic of skin disease-associated connexin 31 mutations.
Di WL, Monypenny J, Common JE, Kennedy CT, Holland KA, Leigh IM, Rugg EL, Zicha D, Kelsell DP
Human molecular genetics 2002 Aug 15;11(17):2005-14
Human molecular genetics 2002 Aug 15;11(17):2005-14
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of WiDr cell lysate (35 ug/lane) with GJB3 polyclonal antibody (Cat # PAB3584) at 2 ug/mL.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GJB3 polyclonal antibody (Cat # PAB3584) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
