GTX116114

antibody from GeneTex

Targeting: ARHGEF3 DKFZP434F2429, GEF3, STA3, XPLN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX116114

Provider

GeneTex

Proper citation

GeneTex Cat#GTX116114, RRID:AB_10622372

Product name

ARHGEF3 antibody [N1C1]

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

MicroRNA-34a modulates c-Myc transcriptional complexes to suppress malignancy in human prostate cancer cells.

Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Deng G, Dahiya R
PloS one 2012;7(1):e29722

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MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma.

Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Chang I, Tanaka Y, Gupta A, Dahiya R
Carcinogenesis 2012 Feb;33(2):294-300

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: MCF-7 7.5% SDS PAGE GTX116114 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ARHGEF3 antibody [N1C1] detects ARHGEF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ARHGEF3 protein stained by ARHGEF3 antibody [N1C1] (GTX116114) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ARHGEF3(GTX116114) antibody at 1:500 dilution.