AF2276

antibody from Novus Biologicals

Targeting: IL17RD FLJ35755, IL-17RD, IL17RLM, SEF

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2276

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-17RD/SEF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse IL-17 RD/SEF in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human IL-17 RD is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17B R is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Mutations in FGF17, IL17RD, DUSP6, SPRY4, and FLRT3 are identified in individuals with congenital hypogonadotropic hypogonadism.

Miraoui H, Dwyer AA, Sykiotis GP, Plummer L, Chung W, Feng B, Beenken A, Clarke J, Pers TH, Dworzynski P, Keefe K, Niedziela M, Raivio T, Crowley WF Jr, Seminara SB, Quinton R, Hughes VA, Kumanov P, Young J, Yialamas MA, Hall JE, Van Vliet G, Chanoine JP, Rubenstein J, Mohammadi M, Tsai PS, Sidis Y, Lage K, Pitteloud N
American journal of human genetics 2013 May 2;92(5):725-43

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IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling.

Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z
Cell research 2009 Feb;19(2):208-15

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17 RD/SEF in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse IL-17 RD/SEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2276, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17 RD/SEF in HEK293 Human Cell Line transfected with Mouse IL-17 RD/SEF by Flow Cytometry. Detection of IL-17 RD/SEF in HEK293 Human Cell Line transfected with Mouse IL-17 RD/SEF by Flow Cytometry.