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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 66416-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- P2RX4 Monoclonal Antibody (1A5A6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: TDSVVSSVT TKVKGVAVTN TSKLGFRIWD VADYVIPAQE ENSLFVMTNV ILTMNQTQGL CPEIPDATTV CKSDASCTAG SAGTHSNGVS TGRCVAFNGS VKTCEVAAWC PVEDDTHVPQ PAFLKAAENF TLLVKNNIWY PKFNFSKRNI LPNITTTYLK SCIYDAKTDP FCPIFRLGKI VENAGHGFQD MAVEGGIMGI QVNWDCNLDR AASLCLPRYS FRRLDTRDVE HNVSPGYNFR FAKYYRDLAG NEQRTLIKAY GIRFDIIVFG KAGKFDIIPT MINIGS (57-341 aa encoded by BC033826)
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1A5A6
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-Ig (P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 66416-1-IG ( P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66416-1-IG ( P2RX4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66416-1-IG ( P2RX4 Antibody) at dilution of 1:400 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66416-1-IG ( P2RX4 Antibody) at dilution of 1:400 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.20 µg Anti-Human P2RX4 (Product # 66416-1-IG, Clone:1A5A6) (red) or 0.20 µg isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1,000. Cells were fixed with 90% MeOH.
