PA5-27123

antibody from Invitrogen Antibodies

Targeting: BCL7A BCL7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27123

Provider

Invitrogen Antibodies

Product name

BCL7A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HeLaS3, Neuro2A, GL261.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BCL7A Polyclonal Antibody (Product # PA5-27123). Sample (30 µg of whole cell lysate). A: HeLa S3.15% SDS PAGE. BCL7A Polyclonal Antibody (Product # PA5-27123) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BCL7A Polyclonal Antibody detects BCL7A protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract.12 % SDS-PAGE. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BCL7A Polyclonal Antibody detects BCL7A protein at nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BCL7A protein stained by BCL7A Polyclonal Antibody (Product # PA5-27123) diluted at 1:1,000. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

BCL7A Polyclonal Antibody detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. BCL7A Polyclonal Antibody (Product # PA5-27123) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A (Product # PA5-27123) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.