- PA5-29326 - Invitrogen Antibodies HK2 antibody

Submitted references circATP2A2 promotes osteosarcoma progression by upregulating MYH9.

Cao X, Meng X, Fu P, Wu L, Yang Z, Chen H
Open medicine (Warsaw, Poland) 2021;16(1):1749-1761

Tanshinone IIA inhibits glucose metabolism leading to apoptosis in cervical cancer.

Liu Z, Zhu W, Kong X, Chen X, Sun X, Zhang W, Zhang R
Oncology reports 2019 Nov;42(5):1893-1903

BACH1 Stabilization by Antioxidants Stimulates Lung Cancer Metastasis.

Wiel C, Le Gal K, Ibrahim MX, Jahangir CA, Kashif M, Yao H, Ziegler DV, Xu X, Ghosh T, Mondal T, Kanduri C, Lindahl P, Sayin VI, Bergo MO
Cell 2019 Jul 11;178(2):330-345.e22

Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression.

Kalainayakan SP, Ghosh P, Dey S, Fitzgerald KE, Sohoni S, Konduri PC, Garrossian M, Liu L, Zhang L
Scientific reports 2019 Feb 5;9(1):1405

Hexokinase 2 drives glycogen accumulation in equine endometrium at day 12 of diestrus and pregnancy.

Bramer SA, Macedo A, Klein C
Reproductive biology and endocrinology : RB&E 2017 Jan 5;15(1):4

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Hexokinase II using 30 µg of IMR32 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Hexokinase II polyclonal antibody (Product # PA5-29326) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Hexokinase II using 20 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Hexokinase II polyclonal antibody (Product # PA5-29326) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Hexokinase II was performed by loading 20 µg of mouse endometrium whole tissue lysate, human endometrium whole tissue lysate and 20 µg of horse endometrium whole tissue lysate per well onto an SDS PAGE gel. Proteins were transferred to a nitrocellulose membrane, and blocked in 3% milk in TTBS membrane. The blot was probed with a 1:5000 dilution of Hexokinase II Antibody (Product # PA5-29326), followed by a 1:10,000 dilution of a HRP conjugated secondary antibody. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF7 (Lane 2), Caco-2 (Lane 3), HCT 116 (Lane 4), HT-29 (Lane 5), U-87 MG (Lane 6), L6 (Lane 7), A-431 (Lane 8) and Hep G2 (Lane 9). The blot was probed with Anti-HK2 Monoclonal Antibody (Product # PA5-29326, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 102 kDa band corresponding to HK2 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of HK2 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a HK2 Polyclonal Antibody (Product # PA5-29326) at a dilution of 1:10000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HK2 Polyclonal Antibody (Product # PA5-29326). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Hk2 antibody HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using HK2 Polyclonal Antibody (Product # PA5-29326). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of HK2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:2,000. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of HK2 was performed in paraffin-embedded rat kidney tissue using HK2 Polyclonal Antibody (Product # PA5-29326) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Paraffin) analysis of HK2 was performed in paraffin-embedded rat heart tissue using HK2 Polyclonal Antibody (Product # PA5-29326) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of HK2 was performed in paraffin-embedded rat stomach tissue using HK2 Polyclonal Antibody (Product # PA5-29326) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:2,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:2,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:2,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. Hexokinase II stained by HK2 Polyclonal Antibody (Product # PA5-29326) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. HK2 Polyclonal Antibody (Product # PA5-29326) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HK2 Polyclonal Antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. HK2 Polyclonal Antibody (Product # PA5-29326) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Immunohistochemical analysis of HK2 protein distribution in equine endometrium at estrus, and Day 12 of diestrus and pregnancy. Small inserted panels show glands located in the stratum compactum. No staining was observed in the negative control (not shown)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 circATP2A2 promoted OS cell malignancy and glycolysis. (a-k) OS cells were transfected with sh-NC or sh-circATP2A2. (a) The interference efficiency of sh-circATP2A2 in OS cells was verified by RT-qPCR. (b-h) The proliferation, cell cycle progression, migration, and invasion of OS cells were evaluated by MTT, plate clone, flow cytometry, and transwell assays, respectively. (i and j) Assessment of glucose uptake and lactate product levels in OS cells. (k) Detection of protein levels of HK2 and PKM2 in OS cells by western blotting. * P < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 circATP2A2 regulated OS cell malignancy and glycolysis by sponging miR-335-5p. (a-j) OS cells were transfected with sh-NC + Anti-NC, sh-circATP2A2 + Anti-NC, or sh-circATP2A2 + anti-miR-335-5p. (a-g) The proliferation, cell cycle progression, migration, and invasion of OS cells were determined by MTT, plate clone, flow cytometry, and transwell assays, respectively. (h and i) Analysis of glucose uptake and lactate product levels in OS cells. (j) Protein levels of HK2 and PKM2 in OS cells were measured by Western blotting. * P < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 MiR-335-5p targeted MYH9 to inhibit OS cell malignancy and glycolysis. (a) The overexpression efficiency of MYH9 in OS cells was detected by Western blotting. (b-k) OS cells were transfected with miR-NC + vector, miR-335-5p + vector, or miR-335-5p + MYH9. (b-h) The proliferation, cell cycle progression, migration, and invasion of OS cells were evaluated by MTT, plate clone, flow cytometry, and transwell assays, respectively. (i and j) Detection of glucose uptake and lactate product levels in OS cells. (k) Analysis of HK2 and PKM2 protein levels in U2OS and Saos-2 cells by Western blotting. * P < 0.05.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 circATP2A2 inhibition decreased OS cell proliferation and glycolysis in vivo . (a and b) Tumor volume and weight of mice injected with OS cells carrying sh-circATP2A2 or sh-NC. (c) circATP2A2 expression in xenograft tumors in the sh-circATP2A2 and sh-NC groups was analyzed by RT-qPCR. (d) The level of Ki67 in xenograft tumors in the sh-circATP2A2 and sh-NC groups was analyzed by IHC. (e) Protein levels of MYH9, HK2 and PKM2 in xenograft tumors in the sh-circATP2A2 and sh-NC groups were detected by Western blotting. * P < 0.05.

PA5-29326

Antibody data